primer3-py Gibbs free energy
I'm using primer3-py0.3.0, the Python bindings for Primer3. I have a sequence "S" that is my candidate primer: I want to see if Gibbs free energy of the last five bases of the primer at the 3' is greater than -9kcal/mol. There is a function "calcEndStability" that "Calculate the 3’ end stability of DNA sequence seq1 against DNA sequence seq2" but I did not understand how to use it. Is there any other way to get Gibbs free energy of the last five bases of a primer?
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marghe08 •
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Do you need to do this with primer3-py? I screen all my primers with IDT's oligoanalyzer personally.