This is a test version of Biostars. For the public version, visit https://www.biostars.org.
mapping transcriptome to genome to find TSS/TES/(+/-)

I have a transcriptome fasta file that looks like this:

>loc.00001.1 gene=loc.00001
TCATTTTCCTGGCATGGATAATTTCGCTGCAAAAGGTGTCGCACCTGAAACAGGAGAAATGCATTAGCAG
CTGTCAAATTACCACGAATTTCGGAGATTATGACGACATTTTAGAATGCGACCAATCGAAACTCGAATCG
AGAAATCTCTATGAGATATTTTCATTTCTCACATTGATAACGGGAATCGAGGAATTCCATGAGCAGAACA
TTTGGATTAAATGCTTTCACGATTTGGTGACCGATTTCATCATCTACATGCCGAAAAGGCTCAAGGATTT

I also have a genome fasta file that is around -500 contigs long.

  > contig_1  
attgacaatttaactcgagcattgttctcgtttacataacattatctcatatgtcctttaccacatctttgtagtttttctggctactcctgggataat

I want to map the transcriptome to the genome such that I can produce a resultant bed file that has the transcript ID, contig ID, TSS, TES, and +/- orientation?

Would I have to use a program like gmap? Would that give me the information I need?

mapping genome alignment

0 answers

No answers yet.

Log in to answer this question.