multiIntersectBed for SNPs, need to compare but not merge intervals

Hello,

I have cases when there are SNPs called adjacent to each other, ex:

scaffold_1  106 107 C/A +
scaffold_1  107 108 G/A +

This is probably because this is not an SNP but an MNP (multiple nucleotide polymorphism?) where more than one bp changes at a time. Regardless, I would like to treat these as SNPs for the time being because they aren't that many and are not affecting my results.

I want to compare different isolate with multiIntersectBed, but the problem I am getting is that bedttools is merging such intervals, creating a common one 106 to 108, which really screws up the analysis. To show you what I mean:

@OHRI-bell-virt /scratch/temp_Test $ head ABWUN.NUMALT1.SNP.bed
scaffold_1  54  55  A/T +
scaffold_1  55  56  G/T +
scaffold_1  62  63  G/A +
scaffold_1  103 104 T/C +
scaffold_1  106 107 C/A +
scaffold_1  107 108 G/A +
scaffold_1  124 125 G/T +
scaffold_1  158 159 A/T +
scaffold_1  162 163 T/C +
scaffold_1  218 219 T/G +

@OHRI-bell-virt /scratch/temp_Test $ cp ABWUN.NUMALT1.SNP.bed ABWUN.NUMALT1.SNP.bed2

@OHRI-bell-virt /scratch/temp_Test $ wc -l ABWUN.NUMALT1.SNP.be*
  458044 ABWUN.NUMALT1.SNP.bed
  458044 ABWUN.NUMALT1.SNP.bed2
  916088 total

@OHRI-bell-virt /scratch/temp_Test $ multiIntersectBed -i ABWUN.NUMALT1.SNP.bed ABWUN.NUMALT1.SNP.bed2 > ABWUN.NUMALT1.SNP.multiIntersect.bed

@OHRI-bell-virt /scratch/temp_Test $ wc -l ABWUN.NUMALT1.SNP.multiIntersect.bed
402231 ABWUN.NUMALT1.SNP.multiIntersect.bed

@OHRI-bell-virt /scratch/temp_Test $ head ABWUN.NUMALT1.SNP.multiIntersect.bed
scaffold_1  54  56  2   1,2 1   1
scaffold_1  62  63  2   1,2 1   1
scaffold_1  103 104 2   1,2 1   1
scaffold_1  106 108 2   1,2 1   1
scaffold_1  124 125 2   1,2 1   1
scaffold_1  158 159 2   1,2 1   1
scaffold_1  162 163 2   1,2 1   1
scaffold_1  218 219 2   1,2 1   1
scaffold_1  225 226 2   1,2 1   1
scaffold_1  239 240 2   1,2 1   1

The fact that the merged file has less intervals (because it merged 106 to 107 and 107 to 108) is creating problems, because I want to compare amount of SNPs present in a given isolate compared to all. When I create a multiIntersect of all isolates and then use it to find out the proportion of SNPs in any given isolate present in at least 1 or 2, I sometimes get more intervals than SNPs in an isolate.

Any idea how to compare with multiIntersect but not merge intervals? Thanks, Adrian

snp bedtools intersect

• 1,890 views • link

updated 9.6 years ago by Alex Reynolds

• written 9.6 years ago by Adrian Pelin

1 answer

You can use bedops and bedmap --count to filter N BED files for at least X mutual exact overlaps:

$ bedops --everything file1.bed file2.bed file3.bed .. fileN.bed \
    | bedmap --count --echo --exact --delim '\t' - \
    | awk -voverlaps=${X} '$1 >= overlaps' \
    | cut -f2- \
    > answer.bed

Set $X to 1, 2, etc. Note that, when counting overlaps in a multiset union, an element will always overlap itself at least once, because it will show up in the multiset union at least once. So a threshold of 2 or more is probably more useful for this counting exercise, generally, unless you want to count everything.

• 0 views • link

written 9.6 years ago by Alex Reynolds