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Convert Illumina 1.5 to Illumina 1.3

I have fastq file in Illumina 1.5 encoding, i want to convert it into Illumina 1.3 encoding.

ngs fastq illumina encoding
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written by Abdul Rafay Khan Abdul Rafay Khan

1 answer

If you want to convert the quality scores from ASCII-33 to ASCII-64, you can do so with the BBMap package like this:

reformat.sh in=x.fastq out=y.fastq qin=33 qout=64

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written by Brian Bushnell Brian Bushnell

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