I have aligned reads with few genes and to view the alignment (bam) file I have loaded those gene sequence to IGV as a custom gene , but unfortunately I could see the alignment with only one gene(note: this gene is smallest in length amongst all) .
I have checked the SAM file to see if the reads has actually aligned properly to these gene or not , the alignment has worked fine and reads were aligned to all the genes, but somehow I am unable to view it in IGV.
I have no idea what could be the reason, any inputs on this ?
What does it mean "I am unable to view it in IGV"? How does it look like? Have you tried zooming?
maybe you need to zoom more?
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