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IGV custom Genome

I have aligned reads with few genes and to view the alignment (bam) file I have loaded those gene sequence to IGV as a custom gene , but unfortunately I could see the alignment with only one gene(note: this gene is smallest in length amongst all) .

I have checked the SAM file to see if the reads has actually aligned properly to these gene or not , the alignment has worked fine and reads were aligned to all the genes, but somehow I am unable to view it in IGV.

I have no idea what could be the reason, any inputs on this ?

igv software error

What does it mean "I am unable to view it in IGV"? How does it look like? Have you tried zooming?

maybe you need to zoom more?

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For this reason I am closing your question.

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@minz: Please don't delete/close posts that have a solution (I have restored the question). This solution is useful for others down the road if they run into same/similar issue.

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1 answer

I figured out what was the problem. The problem was with the sequence header , the header( the sequence identifier) should be same in both the files bam file and genome file loaded in the IGV.

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