This is a test version of Biostars. For the public version, visit https://www.biostars.org.
bcl2fastq to output different lanes together

Hi there,

I need some advice regarding bcl2fastq usage. I have rapind run HiSeq data where clusters were generated on board. All lanes are identical, so we have same the samples with the same barcodes in every lane. Is it possible to join lectures during demultiplexing? I mean, is it possible to put together all reads for every sample coming from different lanes?

Thank you in advance.

Best, Luis

demultiplexing bcltofastq
• 7,528 views • link
updated by GenoMax GenoMax • written by luisantome • luisantome

1 answer

Assuming you are running bcl2fastq v.2.18 use --no-lane-splitting option when you demultiplex.

• 0 views • link
written by GenoMax GenoMax

Log in to answer this question.

More posts like this