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What does the reads per sequences mean

Hi. I'm reading a paper and try to understand a plot of it. In the x-axis there is the position from promoter (bp) and in the y-axis it writes reads per 10^6 sequences.

What exactly that "reads per 10^6 sequences" means?

rna-seq
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written by arronar arronar

That sounds indeed rather cryptic, perhaps you can add from which article this plot is?

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written by WouterDeCoster WouterDeCoster

here is the DOI of the article : doi:10.1038/nature09652

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written by arronar arronar

Oh, that looks like normalized read count to me. Like FPKM but without taking transcript length into account (presumably not required for this).

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written by WouterDeCoster WouterDeCoster

Oh. thank you very much.

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written by arronar arronar

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