pbalign - SortService returned a non-zero exit status
0
0
Entering edit mode
7.5 years ago
mjp ▴ 30

I'm following pbalign tutorial on large datasets. I split my original fofn into subsets containing only three bax.h5 files, there is 14 of them.

I run pbalign as following:

pbalign file_1 reference.fasta out_file_1.cmp.h5 --forQuiver

For every single subset of original fofn I get error message:

[ERROR] SortService returned a non-zero exit status.

Unfortunately there is no more informative messages.

Additionally for one subfile I get different message instead:

[ERROR] FilterService returned a non-zero exit status. Error parsing alignment line 103. Missing or error in field PNEXT

The original movies are about ~1GB in size.

I'm using pbalign as part of smrtanalysis_v2.3.

I went ahead and tested the same approach on just a subset of a movie with:

pbalign -vv file_1.bax.h5 reference.fasta out_file_1.cmp.h5 --forQuiver --algorithmOptions=' -holeNumbers 1-5000 ' --keepTmpFiles

but still got the same error message:

[INFO] pbalign version: 0.2.0.138342
...
[INFO] ForQuiverService: Sort.
[INFO] SortService: Sort a cmp.h5 file using cmph5tools.py.
[DEBUG] SortService: Call "cmph5tools.py -vv sort --deep --inPlace out_file_1.cmp.h5 "
[ERROR] SortService returned a non-zero exit status.

No other messages returned. sam file gets created. Seems to be formatted the right way. Any piece of advice would be appreciated.

pacbio pbalign Assembly • 1.6k views
ADD COMMENT

Login before adding your answer.

Traffic: 2608 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6