Dear all;

I would like to ask you some questions. I want to identify peptidases, neuropeptides and neurotransmitter in a proteome file of a non model organisms.

I did these steps: For peptidases;

I searched proteins in MEROPS database. then I check the results. to confirm each sequence to be able to say that they are peptidases I used PeptideCutter to find cleavage sites.

For Neuropeptides;

I downloaded neuropeptides from uniptrot and did blastp. later, I used SignalP for signal prediction and later I used NeuroPed.

Did I do right?

thank you.