predicting Alternative splicing
I want to predict alternatively spliced exons in human genome. So which dataset i choose i.e RNA seq or Whole exome sequencing. Can anyone tell me.
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Chris Cole
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rajesh
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rajesh
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RNA-Seq. Exome sequencing is sequencing of DNA and not RNA.
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Satyajeet Khare
Using RNA-seq (or exome) data is not a prediction, it is searching for alt-spliced exons. As far as I know there is no reliable method for predicting alt-splicing without RNA data.
Alt-splicing is also tissue and developmental time point dependent so which dataset you use will affect which splice sites you see.
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