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Problem when using Fastqc overrepresented sequences to find adapter

Hi all:

I am totally new to NGS data analysis. Recently I am analyzing the small RNA sequencing data from Illumina devices, in the first trim process, I used fastqc to look into the raw data for adapter seeking, and got the overrespresented sequences result as indicated in below picture. I am totally confused about this result and have no idea which sequence is the adapter. Could you please have a look at it for me?

result

rna-seq next-gen
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written by Sean Liang • Sean Liang

There is a Button Adapter Content, you should check that.

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written by Michael Dondrup Michael Dondrup

Thanks Micheal, I checked the adaptor content and got this information, does this mean the adaptors have already been trimmed?

result

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written by Sean Liang • Sean Liang

This looks good to me. Also, there should not be a standard single end adapter in a small RNA sample. Normally I tend to ignore the overrepresented sequences tab.

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written by Michael Dondrup Michael Dondrup

Thanks a lot for your help Micheal.

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written by Sean Liang • Sean Liang

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