Hi, I had a 7 different genomes (fish) with 40X genome coverage, sequenced by Nextseq-500. 1. I used 3 de novo assemblers (Abyss(21,33,44,77), Spades(21,33,44,77), Velvet(31)) …

Hi, I am doing a denovo assembly with ONT and Illumina reads, for that I'm using Canu assembler first and then, polishing with Illumina reads. …

Hi folks, Does anyone know how pilon treats single N characters? I'm using pilon and some illumina data to polish a pacbio assembled genome with …

Hi all, This is maybe a trivial question, but I can't find the answer anywere. I'm working with the soft masked reference genome of Vitis …

The fungal genome assembly I've done using SPAdes with single-end reads has stretches of Ns present. I would expect this with paired end reads but …

Hi I am annotating a new plant genome and using the Maker-P pipeline. I have created a custom library using RepeatModeler and the assembled genome …

Hi, I have two genome libraries of a plant with different insert sizes and I assembled both with SOAPdenovo. Now i'm trying to merge the …

I am trying to compare two genomes by aligning them together using lastz. I have used the following command lastz path/to/my/assembled/genome/genome.fa[multiple] path/to/other/species/genome/ref.genome.fa.gz > output.maf this …

Does anyone know of any examples where a previously known complete genome has been assembled de novo using ion torrent reads?

<p>Dear all,</p> <p>I am trying to assemble a genome of size around 80Mb. I have four Illumina libraries of insert sizes 300bps, 1kb, 8kb and …