How to estimate isoform expression of a certain cell type given multiple samples?
I know this question may sound naive, but I'm not a biologist so I would like to know if my idea sounds reasonable to the biology community.
If I have multiple RNA-seq samples from the same cell type, does it make sense to say that I want to estimate the isoform expression levels in this cell type? All the current methods I know perform estimation on each sample separately. What if I want a final answer for the cell type, not a set of values for each sample? Can I assume samples from the same cell type have the same isoform expression, ignoring the sequencing biases?
Thanks in advance!
1 answer
Check this post
Log in to answer this question.
More posts like this
-
Wilcox test using data slot (log normalized count values) of scRNA-seq between two groups
written by mropri
-
Calculating percentage of cells proliferating in single cell rna seq data
written by mropri
-
Comparing relative expression of a conserved gene between distantly related species.
written by georgii.vdovin •
-
Deconvolution RNA-seq based on Single-RNA-seq?
written by star
-
single cell analysis: normalize gene expression across samples
written by neta.zuckerman •
-
Using a normalization gene in differential expression analysis
written by joel.tuberosa •
-
How to quantify noise level of estimated isoform expression levels from RNA-seq data ?
written by jack
-
In TCGA RNA-seq exon specific data, why not all the exons of a particular isoform have similar read…
written by ruhulmb •
-
Differential expression in edgeR with cuffdiff count output
written by mjg •
-
Cell-Specific Expression Versus Whole Tissue Expression
written by pld
