I knew that there was bias in the first few bases produced by random priming, but that bias should only affect the fragment position in the genome rather than the fragment sequencing quality
I am running Mutect2 (GATK v4.x) on ctDNA sequencing data generated from Illumina NovaSeq X Plus. NovaSeq X Plus produces discrete base quality bins (Q9, …
Hello everyone, I have some new Illumina datasets that show non-uniform coverage (last image). My older datasets have uniform coverage across the whole genome (first …
Hello, I've been trying to assembly Illumina paired-end reads (2x100bp) from RNA-Seq, but after checking FastQC results I noticed a certain pattern in the first …