qRTPCR internal (house keeping) control for microRNA
I want to study expression of microRNA in human cells and wonder what would be the internal controls for normalization of such data. Can I use b-actin or other regular house keeping genes as internal normalization?
Thanks
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Hi!
It is not the best control to use since you can't pre-process it like the microRNAs. Instead, you should test a few small stable RNAs like the one from the spliceasome (U6 is a classical), ribosomal RNA (5S RNA) or even some tRNA or stable miR instead.