simple explanation needed
How would you explain to an 8-yr old why the log2 value output from DESeq2 is not simply the log2 of the averaged HTSeq count ratio ? Not that I expect 8-yr olds to be using DESeq2, but maybe your boss who is unfamiliar with differential expression methods and shows some scepticism towards the analysis.
An example,
Gene A HTSeq counts control 3 samples - 9, 10, 11
Gene A HTSeq count treatment 3 samples - 48, 50, 52
Gene A DESeq log2 - 1.0
A five fold change in read count, but only 2-fold change in the log2.
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mccormack •
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