Finding Novel Transcripts From A De Novo Transcriptome Assembly
I've assembled a de novo transcriptome from RNAseq data. I'm comparing it against a reference cDNA set that is publicly available. However, I'd like to see if my assembly has produced any novel transcripts. Is there something like a reverse alignment? I'd like to find transcripts that I've assembled that are not included in this file of cDNA. I will then map them to the genome using blat or a similar tool.
How can I do what seems like the inverse of an alignment?
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Nicolas Rosewick
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ilovepython •
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ilovepython •
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Check here :
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zx8754
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Nicolas Rosewick
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Nicolas Rosewick
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