Is TCGA PRAD RNA-seq data strand specific?
I have been trying to find if TCGA PRAD (prostate cancer) RNA-seq data strand specific or not. I can not find any relevant info. I did find that it is Illumina protocol, but nothing more. Does anyone if they are strand specific? Do you have the right link? Thanks
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halffedelf •
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halffedelf •
2 answers
The short answer is no.
The specific RNA-Seq library preparation kit TCGA used actually "could" support stranded library; however, it was used in a manner that libraries are actually strandless.
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at the top of my head I don't know, however, there is a similar questions here on Biostar if you can find out the library preparation method/kit that might help.