Best way to present the single cell RNA-seq data and bulk RNA-seq expression data together
Hi,
Do you have any ideas to better represent single cell RNA-seq data and bulk RNA-seq expression data together?
I have expression values of genes (in RPKM) from single cell RNA-seq data for human developmental stages (6 samples). Another sample or condition of expression data of genes (RPKM) from bulk RNA-seq data and want to represent two data together as a heatmap or any better visualisation. The issue here is that there are several genes with zero RPKM or less than 0.1 RPKM in single cell RNA-seq data and comparatively values are larger in bulk RNA-seq data.
Can we do any scaling or normalization so that values are in the same range or comparable.
Thank you.
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Naresh D J •
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Hi,
Did you find the good way to do this? Thanks.
HY