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bigwig file in UCSC custom track

Hi

I had a wiggle file in mm9 format. I converted it to bedgraph and using UCSC liftovered it to mm10. Then sort it and convert it to bigwig and put it in UCSC custom track. But something was changed. In wiggle format I could see the coverage by height but know all points within genome have same hight. How I could change it?

Thanks in advance

ucsc bigwig rna-seq sequencing
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updated by Sukhi Singh Sukhi Singh • written by Ati • Ati

1 answer

I think you loose this information while using liftOver. It would work fine with a bed file for example and then you can create a bedGraph and later wig or bigwig out of it.

You can try CrossMap so see if you retain it.

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written by Sukhi Singh Sukhi Singh

Thanks a lot. It works

• 1 views • link
written by Ati • Ati

Great, you can mark the answer accepted then (Tick in the round box)

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written by Sukhi Singh Sukhi Singh

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