Why blast result gives Homo sapiens BAC clone
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8.0 years ago
chen ★ 2.5k

Blast results usually give Homo sapiens BAC clone.

For example, following is a sequence from EML4:

AGGTAGACTGAGGTTCTGTAAAGGATCTAGAGAAGTTTATTTGAAAATAAGAAGCATTGCAAATGGCAGTAGTGAAAAAGGTTGGAAAAGAG

Blast it in NCBI, it doesn't give EML4, but only gives:

Homo sapiens BAC clone RP11-379A23 from 2, complete sequence

blast BAC clone • 4.8k views
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As @Jean-Karim pointed out below restricting the search space to "Human genomic + transcript" produces two matches which appear to be the correct result (EML4 isoform a/b).

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8.0 years ago

It depends on which database you're using but most likely your sequence is from an intron. Try using the human genomic DNA database and see where the sequence maps.

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