Why blast result gives Homo sapiens BAC clone
Blast results usually give Homo sapiens BAC clone.
For example, following is a sequence from EML4:
AGGTAGACTGAGGTTCTGTAAAGGATCTAGAGAAGTTTATTTGAAAATAAGAAGCATTGCAAATGGCAGTAGTGAAAAAGGTTGGAAAAGAG
Blast it in NCBI, it doesn't give EML4, but only gives:
Homo sapiens BAC clone RP11-379A23 from 2, complete sequence
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Jean-Karim Heriche
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1 answer
It depends on which database you're using but most likely your sequence is from an intron. Try using the human genomic DNA database and see where the sequence maps.
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Jean-Karim Heriche
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As @Jean-Karim pointed out below restricting the search space to "Human genomic + transcript" produces two matches which appear to be the correct result (EML4 isoform a/b).