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heterozygous chromatogram data

hello, i generated its heterozygous chromatogram of a abi file , in this file i want to know the each peaks confidence score or its quality values at each base in the sequence

genome sequencing
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updated by Benn Benn • written by kapil.joshi036 kapil.joshi036

Sanger sequencing?

If it is, I don't think those have confidence scores.

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written by jotan jotan

ya sir, sanger only then plz tell to get the values of both bases in heterozygous peak

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written by kapil.joshi036 kapil.joshi036

Hello kapil.joshi036!

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PS: duplicate, same author, same question
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written by Pierre Lindenbaum Pierre Lindenbaum

respected sir, i m not getting answer for this query last post i got less views so i reposted it

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written by kapil.joshi036 kapil.joshi036

1 answer

It seems like you asked this before in a previous post ab1 file nucleotide confidence score

I think besides a Phred score there is no other 'confidence' or 'quality' score for the other traces in the file, but you can of course extract the raw data from the files (see Exporting Raw Trace Data ), and make your own quality or confidence score.

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written by Benn Benn

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