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P VALUE FOR MICROARRAY DATASET

I have a microarray dataset of 22998x215 (rows and columns). I want to eliminate the differentially expressed genes out of that 22998 genes. Please help to find the p value and elimination methodologies.

215 - persons (samples) 22998 genes (gene1, gene2 , gene3,..... gene22998)

Here rows represents genes and column represents person. Need to eliminate the insignificant genes out of 22998.

r genome
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updated by Whoknows Whoknows • written by vickygjvignesh • vickygjvignesh

Hello vickygjvignesh!

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written by Michael Dondrup Michael Dondrup

1 answer

Are you working on analyzed data or raw data?? If you have analyzed result you could simply filter P-value < 0.05 or even better feature FDR < 0.05.

But for raw data you will need analysis before obtaining significant genes.

cheers

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written by Whoknows Whoknows

Working on Raw Data taken from ncbi .. need guidance for these 1. How to Read excel data in R (Limma package) 2. How to compute P value for each row

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written by vickygjvignesh • vickygjvignesh

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