What Ngs Read Simulators Are Available For Single-End 35Base Pair Length Data?
I want to generate small RNA data for illumina.
Can anyone suggest the tool to simulate 35 basepair length data for illumina in fastq or fasta format?
I have tried ART (set of simulation tools). But the mapping data is not as expected for small RNA data.
1 answer
Take a look at previous thread,[?]
NGS:Simulation tool, available here Galaxy[?]
There are several other tools availble for fastq simulation:[?]
Mason[?]
simulateSeq.pl[?]
Ilia
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Can you explain "mapping data is not as expected for small RNA data"?
More specifics on how you generated the data are warranted, probably.
You can use the NGS:Simulation tool, available here Galaxy
@pascal: only 1 or 2 reads mapped on the complete genome.I would say there wasnt any mapping...!! @Sean Davin: According to me atleast 3 reads should map onto mature miRNA to prove that it is present!!