Hi,

I would like to simulate a time series small RNA-Sequencing experiment. I guess this would be possible using rlsim and simNGS in combination. However, is anyone aware of any 'runfile' for simNGS for small RNA-Sequencing experiments (like 36 bp reads)?

Or can anyone recommend any other tool out there that can do this given a set of transcripts to simulate reads from, or a genome and annotation file? Possibly with custom abundances as well.

Thanks.