Hello, I develop blast application and I want to send my query to NCBI server "remote". so, I develop Perl file that send a command to blastn.exe.
The command is:
$ProgramPath\\blastn.exe -task blastn -db \"$databaseName\" -query $dataPath/testinput.txt -remote -out $dataPath/testoutput.out
Where $ProgramPath=path to .exe files $databaseName = refseq_rna $dataPath = path to text files "input - output" Now, I got these error:
Error: (301.23) [CONN_Read(blast4/HTTP)] Unable to read data: Timeout[30.000000]
Error: (301.23) [CONN_Read(blast4/HTTP)] Unable to read data: Timeout[30.000000]
Error: NCBI C++ Exception:
"..\..\..\..\..\src\serial\objistr.cpp", line 518: Error: byte 188696: Frame type= eFrameClassMember, Member name= total-length ( at Blast4-reply.body.get-databases[])
Error: (311.26) SOCK#502000[1144]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=10060,Operation timed out}
Error: (303.7) [URL_Connect] Socket connect to www.ncbi.nlm.nih.gov:80 failed: Closed
Anyone can help me with this please?
Thanks,
2 answers
There is a problem with your internet connection, maybe you connect to the internet through a proxy.
Check your proxy settings and internet connection.
Since previous posts posted about where the problem likely comes from, here is a post providing at least some direction to resolve the issue: Howto use NCBI programs behind a HTTP proxy
At least, that worked for me after getting the respective information, i.e., the HTTP_PROXY_HOST and HTTP_PROXY_PORT which will depend on your universities/institutes configuration.
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