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Identifying fixed differences from multi-locus DNA polymorphism data

I have two groups (e.g. pop1 and pop2) of DNA sequences for many loci and I want to know if any have fixed differences (i.e. pop1 has all A and pop2 has all G for a given site, sensu Hey 1991). Is there any open source code or software (besides DNAsp) that can do this for me? Any suggestions would be greatly appreciated.

dna snp alignment

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