Hi All, Here is the question.
Considering the cost and the achievement of the methylation sequencing, Do you think single-end RRBS or BS-seq would be better than pair-end RRBS or BS?
Here is my reason:
- There would be overlap between pair-end sequencing, therefore, loss would be happened here.
- Pair-end alignment process would bring large number useless mapping/reads
But maybe pair-end RRBS have higher creditable result since the alignment accuracy would higher than single-end RRBS.
Am I right? Any comments? Is there any strategy to quantitatively assess this question?