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Forum: Gallery: Computational tools for DNA methylation

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  • BeadScan: Analysing data from Illumina BeadArrays
  • BisReadMapper: Library-free methylation sequencing with bisulfite padlock
  • BRAT: Highly configurable and well-documented three‑letter bisulphite aligner
  • BSMAP: Probably the most widely used wild-card bisulphite aligner
  • BSmooth is a pipeline for analyzing WGBS data: aligning, quality control, and identifying DMRs.
  • ComBat: R script for correcting known or suspected batch effects
  • DMAP: differential methylation analysis package for RRBS and WGBS data.
  • GenomeStudio: GenomeStudio Methylation Module v1.8
  • GSNAP: Wild-card bisulphite aligner included in a widely used general-purpose
  • isva: R package for batch effect correction using an algorithm
  • MethylCoder: Three‑letter bisulphite aligner that can be used with either Bowtie and Bowtie2
  • methylumi: R/Bioconductor package for Infinium data normalization
  • minfi: R/Bioconductor package for Infinium data normalization, analysis
  • Pash: Wild-card bisulphite aligner included in a general-purpose alignment tool
  • RMAP: Wild-card bisulphite aligner included in a general-purpose alignment tool
  • RnBeads: R package providing a software pipeline for Infinium data normalization,
  • RRBSMAP: Variant of BSMAP that is specialized on reduced-representation
  • segemehl: Wild-card bisulphite aligner included in a general-purpose alignment tool
  • SVA: R/Bioconductor package for correcting batch effects
  • methyLiftover: cross-platform DNA methylation data integration
  • dariober's methylation-cafe: https://github.com/dariober/bioinformatics-cafe
  • metilene for DMR (Fast and Sensitive: better than MOABS and Bsmooth) http://www.bioinf.uni-leipzig.de/Software/metilene/Downloads/
  • scLVM: single-cell RNA-seq heterogeneity source deconvolution
  • methpipe: http://smithlabresearch.org/software/methpipe/
  • Bis-SNP: http://people.csail.mit.edu/dnaase/bissnp2011/
  • COMETs: Information recovery from low coverage whole-genome bisulfite sequencing
  • HMMcopy: copy number estimations for whole genome data with GC and mappability correction
  • BS-SNPer: SNP calling in bisulfite-seq data
computational-tools methylation
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updated by Ram Ram • written by Shicheng Guo Shicheng Guo

10 answers

metilene (Link)

References:

  • Jühling F, Kretzmer H, Bernhart SH, Otto C, Stadler PF, Hoffmann S., "metilene: fast and sensitive calling of differentially methylated regions from bisulfite sequencing data.", Genome Res. 2016 Feb;26(2):256-62.
  • Kretzmer et al., "DNA methylome analysis in Burkitt and follicular lymphomas identifies differentially methylated regions linked to somatic mutation and transcriptional control.", Nat Genet. 2015 Nov;47(11):1316-25.
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written by David Langenberger David Langenberger

DNA methylation needs 5 stages: Mapping and Normalization Preprocessing Analysing Visualisation Validation and interpretation Partek company has a software for all these stages but it is not free. My question is: 1- Which of the current packages have the five stages with different options? 2- I need a tutorial to follow to reproduce some figures like? Thank you

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written by forever forever

Bismark?

BiSeq (under R)

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updated by Ram Ram • written by Antonio R. Franco Antonio R. Franco
  • dna-bison - Bisulfite alignment On Nodes of a cluster
  • NextGenMap - Has a methylation specific mapper
  • bwa-meth - Align BS-Seq reads and extract methylation without intermediate temp files
  • BiQ analyser - Easy visualization and quality control of DNA methylation
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updated by Ram Ram • written by Sandeep Sandeep

...Since this thread as returned to the top, here's my 2p.

I wrote a simple parser to extract methylation from bam files, bam2methylation.py

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written by dariober dariober

The latest version of BarraCUDA (ie 0.7.107f http://sourceforge.net/projects/seqbarracuda/, effectively BWA on CUDA hardware) can be used with epigenetics NGS data on graphics cards. However note that currently epigenetics data effectively doubles the size of the memory needed for the reference genome, so for human data you may need a GPU with more than 6GB. For noisy data, you might want to increase the allowed number of mismatches (option -n). Bill

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written by william.bill.langdon • william.bill.langdon

New technical report RN/16/10 https://doi.org/10.1101/095075 gives BarraCUDA performance on nvidia Titan X (pascal), GTX 1080 and Titan X (compute level 5.2) graphics cards on paired end Cambridge Epigenetix data. Bill

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written by william.bill.langdon • william.bill.langdon

BioData Mining has just published our "Short Report" on Genetically improved BarraCUDA. Bill

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written by william.bill.langdon • william.bill.langdon

cgmaptools, a collection of programs to handle methylation data

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written by Antonio R. Franco Antonio R. Franco

For Analysis: MethylKit , For data visualization: SeqMonk

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written by Naz • Naz

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