Hi all! I'm building my first pipeline for human exome variant calling, and I'm starting to learn the basic working principles of genome/exome data analysis. …

Hello! What is the difference between hybrid capture sequencing and whole exome sequencing? It seems that both are target sequencing technology and I cannot understand …

Hello all, I have some exome sequencing data which used an exome capture kit (http://emea.support.illumina.com/downloads/nextera-rapid-capture-exome-v1-2-product-files.html) from Illumina which is based on hg19 coordinates. I aligned …

Hi guys, We have exome sequenced samples at a minimum of 15X coverage using capture kits. - We would like to * identify genes that …

In exome sequencing, specific interval list (-L) is used by each company to capture particular region. But all exome in the genome will be in …

I want to just give a snapshot to type of sequencing and their main use. This post is for beginners who are into NGS data …

Hi friends, Recently, we performed exome sequencing for 3 samples using Nextera sequencing machine. We used new kit for exome sequencing. So I am interested …

I need to understand the difference between complex disease and cancer in terms of variants detection. Can someone please explain briefly how detection of causal …

Hi Guys, I would like to know the Differences/similarities (particularly in terms of Genotyping) between Complete genomics, Whole Exome Sequencing and Sequenom MassArray. Could you …

Dear All, I would like to ask you about an issue am facing. While doing exome sequencing I earlier did an estimation to what extent …