Hi All,
I want examine different gene expression for different treatment by using qRT-PCR, I will not do any sequencing or assembly. First RNA will be extract and then my question is How can I choose the candidate genes before run PCR? Then I can design the primer for the candidate and run PCR.
I hope if someone can explain and suggest methods for chose the candidate.
Thanks
•
written
by
loly.pearl86 •
1 answer
Every gene is a candidate until you can specify what question you want to ask of which animal and which tissue. In a muscle, a muscly-gene is important, and in glands some metabolic genes are interesting.
Your question is like a child learning to add and subtract and asking which numbers to try it on. All the numbers are good.
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