Tool:Multiplex PCR Primer Design Tools

Question

[Shicheng Guo]

This page were applied to list all the computational tools for multiplex PCR primer design for Oligo Design for Multiplex PCR & High Throughput SNP/RNA/BSP and Analysis.

1. http://www.premierbiosoft.com/primerplex/index.html
2. https://omictools.com/msre-htprimer-tool
3. https://omictools.com/multiplex-primer-design-tool
4. https://omictools.com/autodimer-tool
5. https://omictools.com/multiplx-2-tool

Later, I will compare all of them and give some suggestion to the advantage and disadvantage for each other. Of course, If someone already did this job, please give some suggestions.

[WouterDeCoster]

Looking forward to your comparison!

Answer 1

[Shicheng Guo]

Update:

1. UCSD. Prof. Zhang's ppD: http://genome-tech.ucsd.edu/public/Gen2_BSPP/ppDesigner/ppDesigner.php
2. PUMCH, Prof. Li's methprimer: http://www.urogene.org/methprimer2/tester-invitation.html
3. PCRTiler v1.42 : http://pcrtiler.alaingervais.org/PCRTiler/
4. JCVI Primer Designer: https://sourceforge.net/projects/primerdesigner/
5. PrimerSuite: http://www.primer-dimer.com/help/