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identify circular RNA without reference genome

Hello everybody, in general, circular RNA are identified with reference genome. now I want to identify circular RNA without reference genome, Do you have any paper recommended? Thanks a lot!

rna-seq

I think that is a good topic for a research project.

thanks 5heikki,don't need assemble? just use raw data? I need some paper to study. Do you have any paper recommended? Thanks a lot!

Input is assumed to be assembled contigs/transcripts

thanks 5heikki, Do you have any assemble tools recommended? Thanks a lot!

I think Trinity is quite popular at the moment for RNAseq.

1 answer

A blastn based approach I wrote some time ago..

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