unannotated SNP ids
Hi all,
I have a list of unannotated SNPs, I want to find those genes which are present 10kb upstream and downstream of that unannotated SNP.
Thanks in advance
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2 answers
If I've understood correctly your question, I'd suggest you the following workflow:
- Take the positions of those unannotated SNP.
Create a bed file with:
SNP_chromosome SNP_position-10000 SNP_position+10000- Create gene
.bed|.gtf|.gff3file with gene coordinates. - Intersect (
bedtools intersect) these two files.
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You can use the Ensembl VEP for this. To get variants 10kb up and downstream you'll need to alter the upstream/downstream distance to 10kb, which you'll find in the options.
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see How To Map A Snp To A Gene Around +/- 60Kb ?