distribution of read-count per gene for results by HTSeq-count
Hi all:
After running HTseq-count to get read counts for each gene, typically how many counts would you expect for a gene?
In my result for Drosophila, the median of read count per sample is only ~1.
Do you think my result is reasonable?
Thanks in advance!
1 answer
There's no single value that one should expect, it's entirely dependent upon (A) the sequencing depth and (B) the cell type. In general, I would expect the median to be quite low.
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Devon Ryan
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