error in command
/trinityrnaseq/util/align_and_estimate_abundance.pl \
--transcripts reference.fasta \
--seqType fq \
--left /home/file/read1.fastq \
--right /home/file/read2.fastq \
--est_method RSEM \
--aln_method bowtie \
--trinity_mode
above command i used but i found error given below (lines folded for readability)
rsem-calculate-expression --paired-end -p 4
--no-bam-output --bam bowtie.bam
/home/reference.fasta.RSEM
RSEM died with ret: 65280 at /trinityrnaseq/util/align_and_estimate_abundance.pl line 632
any body can find the what actually it needs?
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Maybe its a silly comment but, are you sure that your left and right files have read1.fastq and data2.fastq names, respectively?
sory its read1.fastq and read2.fastq
Looks like something is missing at the end - errors usually include a line number after the "
xyz.pl line" partHi, I know this was posted almost two years ago, but I have the exact same input and error! Were you ever able to figure it out?