Hi, My name is Kenneth.
I found that mm10.p4 and mm10 the total length of sequence is different in websites. (NCBI, Ensembl ..)
http://www.ebi.ac.uk/ena/data/view/GCA_000001635.6
http://www.ncbi.nlm.nih.gov/assembly/GCF_000001635.24
http://www.ncbi.nlm.nih.gov/assembly/327618
mm10.p4:
Total length: 2,803,568,840
Ungapped length: 2,724,212,084
mm10:
Total length: 2,793,712,140
Ungapped length: 2,714,420,385
I downloaded the FASTA files and counted ACTGN bases, resulted in the number of Nucleotides are same between mm10.p4 and mm10.
But why the "Total length", "Ungapped length" are different between mm10.p4 and mm10?
2 answers
See information about genome build "patches" here: http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/info/patches.shtml
Mouse genome overview: http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/mouse/
From my understanding, total number of nucleotides may be different. But, the number of nucleotides on the major chromosomes (chr1, chr2, ..., chrX, chrY) should be kept the same for the same version reference.
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