Single-cell RNA-seq : for what questions ? and what are the classical pipelines ?
Hello stars!
I'm trying to find out a little bit about single cell RNA-seq and I want to know in which context (the major problems that this technique can answer) and what are the pipelines used in general when we have both classical RNA-seq AND single-cell RNA-seq data?
Thanks in advance
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schmilblik •
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schmilblik •
2 answers
Hi
This recent review might be useful to you..
- STAR: ultrafast universal RNA-seq aligner
- TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
- Cufflinks: Transcriptome assembly and differential expression analysis for RNA-Seq.
- Trinity: a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data
- SpliceMap: SpliceMap is a de novo splice junction discovery tool
- Degust: User friendly RNA-seq differential expression analysis with Degust
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Shicheng Guo
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Shicheng Guo
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