You will need to provide a lot more details to get an informative answer to your question. What sequencing technology are you using? Which formats are your reads in? Were your reads pre-processed?
I think you are talking about adapter sequence, and possibly you are using 454? We shouldn't have to guess though, so I recommend you tell us all the facts. Just in case, adapter sequences will be clipped automatically when converting sff to fasta using the gs* tools coming with the roche sequencer.
When the genome was submitted, NCBI reported a problem: >We ran your sequences through our Contamination Screen. The screen found contigs that need to be …
Hi, I'm trying to get variants from amplicon-based sequencing reads. These reads have: primer adapters and barcodes on both ends. I'm looking into the GATK …
<p>Experiment: deep sequencing for mutants in 700nt fragment.</p> <p>the fragment of dna was preamplified by primers flanking the fragment followed by hiseq.</p> <p>per base coverage …
You will need to provide a lot more details to get an informative answer to your question. What sequencing technology are you using? Which formats are your reads in? Were your reads pre-processed?
I think you are talking about adapter sequence, and possibly you are using 454? We shouldn't have to guess though, so I recommend you tell us all the facts. Just in case, adapter sequences will be clipped automatically when converting sff to fasta using the gs* tools coming with the roche sequencer.