RMBase ( R NA M odification Base ) is a RNA modification database and designed for decoding the landscape of RNA modifications ( >100 different modification types ) identified from thousands of high-throughput RNA sequencing data ( Pseudo-seq, Ψ-seq, CeU-seq, Aza-IP, MeRIP-seq, m6A-seq, miCLIP, m6A-CLIP, RiboMeth-seq) and public modification databases.
It contains ~226000 N6-Methyladenosines (m6A) , ~9500 pseudouridine (Ψ) modifications, ~1000 5-methylcytosine (m5C) modifications, ~1210 2′-O-methylations (2′-O-Me) and ~3130 other types of RNA modifications(e.g. m1A, m6Am).
RMBase demonstrated thousands of RNA modifications located within regulatory RNAs (e.g. mRNAs, lncRNAs, miRNAs, pseudogenes, circRNAs, snoRNAs etc.), miRNA target sites and disease-related SNPs.
By integrating GWAS data into database, RMBase can be used to illustrate the clinically relevant RNA modification sites.
RMBase demonstrated thousands of RNA modifications located within miRNA target sites (~20,000) and RNA-Binding Proteins (RBPs) regions(>1,000,000 items).
This resource and tools will greatly help biologists to explore the prevalence, mechanism and function of various RNA modifications(>100 types) and link them with human cancers and other diseases.
RMBase has been updated to RMBase v2.0.
RMBase Database is freely available at http://rna.sysu.edu.cn/rmbase/.
RMBase continually be maintained and updated whenever novel high-throughput epitranscriptome sequencing data sets are released in public databases.