Split PacBio bax.h5 files

Question

[Coryza]

Hi,

I have a set of 21 bax.h5 files (7 smrt cells) and I was wondering whether someone knows if it is possible to split these bax.h5 files (so lets say I want 84 bax.h5 files instead of 21). This for downstream processing where we run into memory problems.

Thanks..

[GouthamAtla]

I do not know if you can directly split the bax files, but you should be able to covert them to fasta/fastq using pbh5tools and then split. Downstream processing tools should be able to accept fastq/fasta files.

Because the .bax files are associated with .bas files, its complicated to split the files.