Dear All,

I'm an amateur in bioinformatics. I am learning to use qiime for processing my 454 sequencing data. I followed the qiime online tutorial and made some progress.

I want to build a phylogenetic tree with my data. I was able to build a tree with qiime (rep_set.tre) and it can be viewed in Treeview X, but all of the nodes are showing OTU labels (numbers) and nothing about taxonomy or bacteria assignment. Then I looked for other software to build the tree and read some posts said Megan 5 is very powerful. I also read that Megan will take Biom format data from qiime. However, when I tried to import my data (otu_table.biom) to Megan 5, it shows

Execute failed: java.io.IOException: java.lang.IllegalStateException:Expected EGGIN_OBJECT but was STRING at line 1 column 4.

I tried to use another PC, same error.

Please, can anybody help me solve the problem or let me know other ways to build a nice tree.

Thanks in advance!