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ERROR RAISED DURING STEP: Assign taxonomy

Hi

I have installed QIIME and I am going over ''454 Overview Tutorial: de novo OTU picking and diversity analyses using 454 data'' trying to apply each step using data provided in the QIIME website.

I have encountered an error trying to perform ''De novo OTU picking'' section of the tutorial using pick_de_novo_otus.py. I also looked at my directory and I noticed that the file containing Taxonomic Assignment has not been created.

Sorry I am a newbie in Qiime.

* ERROR RAISED DURING STEP: Assign taxonomy

Command run was:

assign_taxonomy.py -o otus/uclust_assigned_taxonomy -i otus/rep_set//seqs_rep_set.fasta

Command returned exit status: 1

qiime assign taxonomy pick_de_novo_otus.py

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