Best 16S Region ?
Does someone have an idea of the best 16S gene region to amplify with NGS in order to assess microbial (bacterial) diversity in environmental samples ? cheers
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Dph •
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You can use information from The Ribosomal Database Project (RDP):[?] http://rdp.cme.msu.edu/ [?] or Silva database[?] http://www.arb-silva.de/ [?]
Ilia
Our colleagues use one 16S amplicon across the V3-V5 region.
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What do you mean best? Do you refere to selecting from among the universal primers? http://en.wikipedia.org/wiki/16S_ribosomal_RNA#Universal_Primers