More posts like this
-
Conversion of VectorBase gff file format to a 12-column BED format for UCSC trackhub viewing
written by ZuelTech •Hello! I have a gff file from Vector Base. I want it to convert it to a 12-column BED file format (http://genome.ucsc.edu/FAQ/FAQformat.html#format1). After converting it …
-
Is there any method to convert a text file to VCF format?
written by Abbas.M •Hello, I want to convert a text file into vcf format, is there any script or any method to convert it into vcf? It is …
-
How to retrieve metadata from the manifest data and UUID from genomic data commons (GDC)
written by a.jamesHello All, I have exon datasets (aligned BAM) files downloaded. I now need the metadata information for the same samples. How can I download or …
-
bedtools bamtobed of mixed paired-end and non-paired datasets
written by tassa.saldi •Hello, I'm wondering if anyone out there can help me. I am trying to calculate mean coverage over exons and introns in my RNAseq dataset …
-
Should you retain all the alignments after primer clipping using Katana for variant calling or just…
written by bioinfo89 •Hello All, I am working with amplicon sequencing data for ~200 samples. For the same, I did soft clipping of gene specific primers using Katana. …
-
extract region bam
written by J.F.JiangHi all, I want to extract bam of specific amplicon to evaluate the according amplicon performance. I used to use samtools view xx.bam chr:start-end to …
-
Convert fastq to plink .bed
written by genomics Newbie •I’m a genomic newbie, so I believe my question is a basic one. I need to transform raw fastq files into plink .bed files. From …
-
Querying positions (bed file) towards BAM files using samtools mpileup
written by a.jamesDear All, I need to query a set of chromosomal positions towards a couple of BAM files, and I am using samtools mpileup for this. …
-
Any tools converting Genbank format to GFF3 format?
written by biolabDear all, As my title describe, I am asking help to convert Genbank format to GFF format. By Googling, I found a perl script (`bp_genbank2gff3.pl`), …
-
Convert Bam To Bed/Fam/Bim
written by Anjali •<p>Hi,</p> <p>I used Bedtools to convert BAM to BED format for my sequencing data. But together with BED I need the corresponding BIM and FAM …
what is Amplicon information ?
The BED file contains a single track of the genomic regions of the expected PCR amplicons for all probes in the design. It also lists the Amplicon ID and strand for each amplicon.
To determine the insert size for inferring the original fragments you can try this:
https://www.cgat.org/downloads/public/cgat/documentation/scripts/bam2bed.html
For amplicon aware variant calling you can use:
https://github.com/AstraZeneca-NGS/VarDict
Or, If you still want to extract the Amplicon location. I am afraid there is no direct solution for that because it is bit complex task. But is is not impossible...you can read this discussion.
Ok..Thank you :)