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BED file with amplicon information

Hello All,

I am looking for a method to convert BAM format into BED format with Amplicon information in it. I mean I need a column which has Amplicon information in it in my BED file

Thanks for any suggestions

rna-seq snp next-gen-sequencing

The BED file contains a single track of the genomic regions of the expected PCR amplicons for all probes in the design. It also lists the Amplicon ID and strand for each amplicon.

To determine the insert size for inferring the original fragments you can try this:

https://www.cgat.org/downloads/public/cgat/documentation/scripts/bam2bed.html

For amplicon aware variant calling you can use:

https://github.com/AstraZeneca-NGS/VarDict

Or, If you still want to extract the Amplicon location. I am afraid there is no direct solution for that because it is bit complex task. But is is not impossible...you can read this discussion.

Ok..Thank you :)

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