issues in genotyping using TaqMan Assay

0

Entering edit mode

9.0 years ago

jfjiang ▴ 10

Hi all,

We have ordered SNP TaqMan assay (MAF=0.38) from Life, and genotyped it for 100 samples.

Although there are 3 clusters, the degree is not good enough to report the genotype for samples.

We asked Life for original QC file showing very good clusters that are so different from ours.

Is there any one know the reason?

BTW, we use EP1 from Fluidigm to perform the genotyping.

Thanks

TaqMan genotyping • 1.6k views

ADD COMMENT • link updated 15 months ago by Ram 43k • written 9.0 years ago by jfjiang ▴ 10

0

Entering edit mode

Could you share the pictures of the clusters?

ADD REPLY • link updated 15 months ago by Ram 43k • written 9.0 years ago by Floris Brenk ★ 1.0k

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