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issues in genotyping using TaqMan Assay

Hi all,

We have ordered SNP TaqMan assay (MAF=0.38) from Life, and genotyped it for 100 samples.

Although there are 3 clusters, the degree is not good enough to report the genotype for samples.

We asked Life for original QC file showing very good clusters that are so different from ours.

Is there any one know the reason?

BTW, we use EP1 from Fluidigm to perform the genotyping.

Thanks

taqman genotyping

Could you share the pictures of the clusters?

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