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How to combine bisulfite sequencing data with Medip seq and/or MRE-seq
I was wondering if anybody has done integration of data from two different sequencing methods for DNA methylation like bisulfite-seq and Medipseq.I would really appreciate if someone could suggest methods for the same. Thanks
dna methylation bisulfite-sequencing medipseq
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updated by Ram Ram • written by Saad Khan Saad Khan

1 answer

Do you have control/treatment data for both?

My suggestion would be to focus on promoter regions +/- 1kbp around TSS and quantify the methylation in each case, %methylation for BS-seq and counts per million for MeDIP-seq, then you can analyse differences with statistical test, merge datasets on gene accession number and perform correlation analysis.

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updated by Ram Ram • written by mark.ziemann mark.ziemann

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