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How to compare correctly a set of tuberculosis DNA gyrase gene region sequences from WGS: resistant versus susceptible to Ofloxacin?

How to compare correctly a set of tuberculosis DNA gyrase gene region sequences from WGS: resistant versus susceptible to Ofloxacin?

What software and concrete packages to choose? What can I figure out except BP content and alignment? Format is fasta. How to compare these two data sets and produce meaningful reports with charts? Need help, thank you. I am not an expert in WGS and sequence analysis. I had 4 years experience with microarray data processing.

sequencing software wgs data

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