CDNA libray 410 peak?!
We are trying to do RNA-seq for mitochondrial and nuclear RNA and we sent the sample to genomics center, they made the library and they found a prominent peak in all of sample around 410bp. Any guess about that?
My samples are from plant
• 1,723 views
•
link
updated
by
Ram
•
written
by
mahnazkiani
•
written
by
mahnazkiani
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Discrepancy Between Fragment Size Distribution and MACS3-Predicted Fragment Length in ChIP-seq Data…
written by christoph.neu •
-
Mitochondrial genes - TPM calculation bulk RNA-Seq
written by nick_b55 •
-
Enrichment of mitochondrial and ribosomal pathways - an artifact?
written by omer.shomrat •
-
Any reason for scRNA data of mouse lung samples to fail to capture epithelial cells?
written by 윤여가(대학원 바이오정보학) •
-
Why is my miRNA-seq mapping rate so low?
written by cecilomar6 •
-
Forum: GENEWIZ RNA-seq quality feedback
written by fmarchildo •
-
Designing the RNA-seq for plant-insect interaction
written by mahnazkiani
-
RNA-seq quality control
written by Ron
-
RNA-seq results do not match qRT-PCR results
written by xiaoyonf •
-
High level of duplicate in one reads of paired-end data
written by olifds •
Sounds like you have some RNA that is 410bp. What was the question?
And how is this bioinformatics? Expecting the question to close soon because it's not a technology question, but a wetlab question.
I'll leave this open for the time being (though I expect someone else will close it) simply to not dissuade someone who happens to have good insight from replying. However, I suspect you'll get better feedback from SEQanswers. The regulars over there tend to bend more toward the wet-lab side of the spectrum than those here.
BTW, it's somewhat important to know at what point in the library prep this was noticed. There are a bunch of rRNAs in plants, so they can show more peaks than you'd see in mammals (presumably that's what the genomics center is used to seeing). If/when you post on SEQanswers, you might attach the whole plot and provide these details.