i want to konw that what does it mean score and bits in local blast output from the following example?'.
Score = 38.2 bits (19), Expect = 0.11
Identities = 22/23 (95%)
Query: 1 cttaacttcagctacctcgagta 23
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Sbjct: 732552 cttaacttcagctacctccagta 732574
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written
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Manju •
See this description of the bit score on NCBI.
Also from this NCBI page:
The bit score gives an indication of how good the alignment is; the higher the score, the better the alignment. In general terms, this score is calculated from a formula that takes into account the alignment of similar or identical residues, as well as any gaps introduced to align the sequences.
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Nikolay Vyahhi
Thank you, Nikolay, for the "obvious" answer. This is a legitimate question, of course, but could have easily been answered by Manju by simply looking at the extensive and useful help pages and other documentation provided on the NCBI pages.
A bit is a measure of information content. Bites, like bytes, are units of measure for digital information, in this case, with regard to the alignment and hence the similarity of the two sequences.
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